ybfM [2019-08-01 14:37:27]

similar to alkaline phosphatase

locus: BSU_02280

pI: 9.48

mw: 18.20 kDa

protein length: 162 aa

gene length: 489 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 248,268 → 248,756

The protein

Protein family

• dedA family (with [protein|289828D37CF5422908316ED2EF1AEAE76F84161D|YngC] and [protein|96CE303E9F757FBEBDEA5ADC8481697E2CD1361A|YkoX], according to Swiss-Prot)

Paralogous protein(s)

• [protein|289828D37CF5422908316ED2EF1AEAE76F84161D|YngC]
• [protein|96CE303E9F757FBEBDEA5ADC8481697E2CD1361A|YkoX]:

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|27FB777AEBCC794A5A768C7FE7D8374BCEA75913|ybfK]-[gene|DE49797486CEC172F912D26A6BF2223190BF5DAD|pssA]-[gene|B2C089DCE51CA0CB11947FCD6F4EFAFF66269829|ybfM]-[gene|F34915697F3EF05E6A683055F46346C73F25CAFC|psd]
  description: [Pubmed|22383849,14762009]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14762009], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|14762009], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

Biological materials

Mutant

• BKE02280 (Δ[gene|B2C089DCE51CA0CB11947FCD6F4EFAFF66269829|ybfM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTAATCCGCTATGAGCTGCT, downstream forward: _UP4_ATCGGAAGGGTTATAGGGAT
• BKK02280 (Δ[gene|B2C089DCE51CA0CB11947FCD6F4EFAFF66269829|ybfM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTAATCCGCTATGAGCTGCT, downstream forward: _UP4_ATCGGAAGGGTTATAGGGAT

References

• 14762009